rlmCD [2019-08-16 10:33:06]

rRNA methyltransferase

locus: BSU_06730

pI: 5.71

mw: 51.65 kDa

protein length: 459 aa

gene length: 1380 bp

function: 23S rRNA maturation

product: rRNA methyltransferase

essential: no

ec: 2.1.1.190

synonyms: yerS,yefA

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.2|rRNA modification and maturation]

Gene

Coordinates: 737,603 → 738,982

The protein

Catalyzed reaction/ biological activity

• methylation of U747 and U1939 in 23S rRNA [Pubmed|21824914]
• S-adenosyl-L-methionine + uridine⁷⁴⁷ in 23S rRNA --> 5-methyluridine⁷⁴⁷ in 23S rRNA + H⁺ + S-adenosyl-L-homocysteine (according to UniProt)
• S-adenosyl-L-methionine + uridine¹⁹³⁹ in 23S rRNA --> 5-methyluridine¹⁹³⁹ in 23S rRNA + H⁺ + S-adenosyl-L-homocysteine (according to UniProt)

Protein family

• [SW|Methyltransferase superfamily] (according to UniProt)
• [SW|class I-like SAM-binding methyltransferase superfamily] (according to UniProt)

Paralogous protein(s)

• [protein|05392BB2C32B0405799D4F34E5BA55FC936AB943|YfjO]

[SW|Cofactors]

• Fe-S cluster [pubmed|29292548]

Structure

• [PDB|2BH2] (from ''E. coli'', 32% identity) [Pubmed|15766524]

Expression and Regulation

Operons

• genes: [gene|943294ACA74067A8549056699E08DF5B82337976|dgkB]-[gene|B97ABE3233A27A7C8273ED9A1E688247DD37FD12|rlmCD]
  description: [Pubmed|22383849]

• genes: [gene|943294ACA74067A8549056699E08DF5B82337976|dgkB]-[gene|B97ABE3233A27A7C8273ED9A1E688247DD37FD12|rlmCD]-[gene|5B5014A68D777DB79179904D5FD3D14DEE0BF1F4|yefC]-[gene|D3F00747006D974A8635D4391E0C261A3276E6AB|dnmA]-[gene|2E55D086DD5023B98929FB8B61238C00E66B8BCB|yeeB]-[gene|DB1AAC91947A6008F631266E5CC85B58703AFD9D|yeeC]
  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-A931 (yefA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/931 NBRP B. subtilis, Japan]
• BKE06730 (Δ[gene|B97ABE3233A27A7C8273ED9A1E688247DD37FD12|rlmCD]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE06730 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTATAATCACCTCTAGT, downstream forward: _UP4_TTAAAAGAATAAATAGTCAA
• BKK06730 (Δ[gene|B97ABE3233A27A7C8273ED9A1E688247DD37FD12|rlmCD]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK06730 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTATAATCACCTCTAGT, downstream forward: _UP4_TTAAAAGAATAAATAGTCAA

References

• 21824914,15766524